Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Co...

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This journal article is previously published as: Liu Huan. (2021). Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Communities along Environmental Gradient. Journal of Environment and Health Science (ISSN 2314-1628), 2021(02). https://doi.org/10.58473/JBS0011, which is converted into Journal of Biological Sciences (ISSN 2958-4035). Both Journals belong to the same publisher, Liu Huan. The previous journal article is closed to the public, but the previous reference is still valid.

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2016. Copyrights Register Information: The majority of these materials are registered as book '著作权人：刘焕；作品：《研究生文凭进展（第三版）》' 2016, which can be cataloged in National Copyright Database: http://qgzpdj.ccopyright.com.cn/

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Formally published on 30/11/2022; Latest revised on 29/05/2023.

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Article 6-4. Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Communities along Environmental Gradient/细胞的生物磁场及血细胞群落在环境梯度上的分离

Author: Liu Huan (1983-), Master of Science (First Class Honours,2009), The University of Auckland. ORCID: https://orcid.org/0000-0003-4881-8509

Methods

Step 1. The host cells (such as blood cells of rat) are abstracted from host body.

Step 2. Electrophoresis of blood cells is conducted in moderate electromagnetism; For the simulation of moderate electromagnetism condition, cells are cultivated in electrophoresis pipe for cell electrophoresis, in which the external electric field is added. Subsequently, the electrophoresis pipe is horizontally placed under external vertical magnetism field imposed by magnetic instrument.

Step 3. Different blood cell communities are separated along the environmental gradient over different electric potentials, leading to cell samples with different immunology, which are collected from different electric potentials in electrophoresis pipe.The cell samples, abstracted from different electric potential (j1, j2...jn), are labeled on the basis of electric potential.

Step 4. The specificity of host-invasion interaction is examined on each cell sample. To achieve this, the targeted pathogens are inoculated separately into each blood cell sample prepared in step 3. It is expected that the specific electric potential corresponds to the host cells with apparent antibiotics against the specific invasive virus (or bacteria), which also becomes the key parameter of biophysical training for the host cells with immunology against the specific invasive virus (or bacteria). Nevertheless, for the mobilizable blood cells, it is expected that the 'ecological niche' of cells vary in their life cycle along this environmental gradient of electromagnetism signal, because of the variation in bio-magnetic field over cell's life cycle, moving from a specific electric potential to another electric potential.

Discussion

Although electrophoresis of blood cells has been used for the pathological diagnose in other people’s research before (such as diagnosing ischemic stroke, coronary heart disease, cor pulmonale, myocardial infarction, hypertension, tracheitis, chronic bronchitis and systemic lupus erythematosus)[2], my research combines the electrophoresis of blood cells with the host-pathogen interaction to identify the specific antibiotics. The bio-magnetic field of blood cells varies even within the same genetic strain, so that different cell communities can be separated according to the gradual variation in electromagnetism signals (environmental gradient of electromagnetism) in this electrophoresis, leading to cell samples with different immunology.

It is expected that the time-varying electromagnetic field of biophysical training is better than constant electromagnetic field, due to the phenotype evolution of invasive virus (bacteria).

This is the revised materials in book “Proceedings for Degree of Postgraduate Diploma in Environmental Science (3rd Edition).” Published in 2016. The ‘chapter’content mentioned in this article is in previous book. Firstly Revised on 05/01/2021; Secondly Revised on 08/02/2021;Thirdly Revised on 29/09/2021. This journal article is previously published as: Liu Huan. (2021). Article 10-4. Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Communities along Environmental Gradient. Journal of Environment and Health Science (ISSN 2314-1628), 2021(02)., which is converted into Journal of Biological Sciences (ISSN 2958-4035). Both Journals belong to the same publisher, Liu Huan. The previous journal article is closed to the public, but the previous reference is still valid. Latest revised on 17/04/2023; 29/05/2023.

References:

[1]. 红细胞电泳。搜狗百科。