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发表于 2021-2-8 17:17:45 | 只看该作者 回帖奖励 |倒序浏览 |阅读模式
This journal article is previously published as: Liu Huan. (2021). Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Communities along Environmental Gradient. Journal of Environment and Health Science (ISSN 2314-1628), 2021(02). https://doi.org/10.58473/JBS0011, which is converted into Journal of Biological Sciences (ISSN 2958-4035). Both Journals belong to the same publisher, Liu Huan. The previous journal article is closed to the public, but the previous reference is still valid.

2023. Copyrights Certificate Registered by Brock Chain Technology: Brock Chain ID. (5cd4ac8ee25e9bdfa122addf2ed842449e1fc979249b8e22a1a03293f6cba4cb) ;

2016. Copyrights Register Information: The majority of these materials are registered as book '著作权人:刘焕;作品:《研究生文凭进展(第三版)》' 2016, which can be cataloged in National Copyright Database: http://qgzpdj.ccopyright.com.cn/

2016. 版权注册信息:本文大多数内容已经以图书形式登记注册在全国版权数据库,登记入库信息:著作权人:刘焕;作品:《研究生文凭进展(第三版)》 2016;可在全国版权登记数据库检索 http://qgzpdj.ccopyright.com.cn/

The formally published serials is the printing <Journal of Biological Sciences (ISSN 2958-4035)>, and the serials NO. is the month/year when the materials is accessible on this website, authorized by publisher;正式发表的期刊是印刷版《生物科学杂志 (ISSN 2958-4035)》,期刊期号为文章内容在本网站上网年/月,出版人许可自行正式发表。

Originality Certificate: The originality of text in English is 98% tested by Turnitin (International). The Turnitin Originality Report is attached in PDF summary.

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Latest revised on 29/05/2023.

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Cited as: DOI:10.58473/JBS0011      Retrieval from official database: www.crossref.org

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Article 6-4. Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Communities along Environmental Gradient/细胞的生物磁场及血细胞群落在环境梯度上的分离

Author: Liu Huan (1983-), Master of Science (First Class Honours,2009), The University of Auckland. ORCID: https://orcid.org/0000-0003-4881-8509

Methods
Step 1. The host cells (such as blood cells of rat) are abstracted from host body.

Step 2. Electrophoresis of blood cells is conducted in moderate electromagnetism; For the simulation of moderate electromagnetism condition, cells are cultivated in electrophoresis pipe for cell electrophoresis, in which the external electric field is added. Subsequently, the electrophoresis pipe is horizontally placed under external vertical magnetism field imposed by magnetic instrument.

Step 3. Different blood cell communities are separated along the environmental gradient over different electric potentials, leading to cell samples with different immunology, which are collected from different electric potentials in electrophoresis pipe.The cell samples, abstracted from different electric potential (j1, j2...jn), are labeled on the basis of electric potential.

Step 4. The specificity of host-invasion interaction is examined on each cell sample. To achieve this, the targeted pathogens are inoculated separately into each blood cell sample prepared in step 3. It is expected that the specific electric potential corresponds to the host cells with apparent antibiotics against the specific invasive virus (or bacteria), which also becomes the key parameter of biophysical training for the host cells with immunology against the specific invasive virus (or bacteria). Nevertheless, for the mobilizable blood cells, it is expected that the 'ecological niche' of cells vary in their life cycle along this environmental gradient of electromagnetism signal, because of the variation in bio-magnetic field over cell's life cycle, moving from a specific electric potential to another electric potential.

Discussion
Although electrophoresis of blood cells has been used for the pathological diagnose in other people’s research before (such as diagnosing ischemic stroke, coronary heart disease, cor pulmonale, myocardial infarction, hypertension, tracheitis, chronic bronchitis and systemic lupus erythematosus)[2], my research combines the electrophoresis of blood cells with the host-pathogen interaction to identify the specific antibiotics. The bio-magnetic field of blood cells varies even within the same genetic strain, so that different cell communities can be separated according to the gradual variation in electromagnetism signals (environmental gradient of electromagnetism) in this electrophoresis, leading to cell samples with different immunology.

It is expected that the time-varying electromagnetic field of biophysical training is better than constant electromagnetic field, due to the phenotype evolution of invasive virus (bacteria).

This is the revised materials in book “Proceedings for Degree of Postgraduate Diploma in Environmental Science (3rd Edition).” Published in 2016. The ‘chapter’content mentioned in this article is in previous book. Firstly Revised on 05/01/2021; Secondly Revised on 08/02/2021;Thirdly Revised on 29/09/2021. This journal article is previously published as: Liu Huan. (2021). Article 10-4. Bio-magnetic field of Cell and Its Application on Separation of Blood Cell Communities along Environmental Gradient. Journal of Environment and Health Science (ISSN 2314-1628), 2021(02)., which is converted into Journal of Biological Sciences (ISSN 2958-4035). Both Journals belong to the same publisher, Liu Huan. The previous journal article is closed to the public, but the previous reference is still valid. Latest revised on 17/04/2023; 29/05/2023.

References:
[1]. 红细胞电泳。搜狗百科。

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