|
|
楼主 |
发表于 2021-9-25 15:09:19
|
显示全部楼层
Article 10-2. The Experiment Procedure for Blood Cell Cultivation in Biophysical Simulation/生物物理实验中血细胞培养方法
Author: Liu Huan (1983-), Master of Science (First Class Honours), The University of Auckland.
Published after graduation on 11/01/2016, Revised on 08/02/2021.
The blood samples of a rat is abstracted and divided into two samples for the bio-signal simulation:
1.There are two kinds of cultivation conditions simulated in Lab for cell division: one is the ‘comfortable’ condition (Sample 1); the other is under electromagnetism simulation for cell cultivation (Sample 2); the cell samples are collected after sufficient cell division (Ten generations).
2.After sufficient cell division process, the electromagnetism simulation stops. Then both sample 1 and sample 2 are separately transferred into the simulation process of physiological saline: cells are cultivated individually in different concentrations of physiological saline in Lab, and different cell environment (salinity stress of cell environment or ‘thirsty’ simulation) are labeled as T1, T2, ..., Tn.
3.Metabolomics tests are conducted in cell samples after simulation process of physiological saline, T1, T2, ..., Tn, respectively, resulting in different zymograms as: M1, M2, ..., Mn. The procedure of the zymograms analysis is described in the first article of metabolomics in this journal [1].
The other procedures are the same as described above.
However, for the comprehensive assessment of immunology in host cells, the simulation process of physiological saline is replaced by the invasion simulation caused by different families of bacteria (or virus):
The blood samples of a rat is abstracted and divided into two samples for the bio-signal simulation:
1.There are two kinds of cultivation conditions simulated in Lab for cell division: one is the ‘comfortable’ condition (Sample 1); the other is under electromagnetism simulation for cell cultivation (Sample 2); the cell samples are collected after sufficient cell division (Ten generations).
2.After sufficient cell division process, the electromagnetism simulation stops. Then both sample 1 and sample 2 are separately transferred into the simulation process of bacteria (or virus) invasion: cells are cultivated individually and independently during the simulation of different families of bacteria (or virus) in Lab, and the invasion simulation process of different bacteria (or virus) families are labeled as T1, T2, ..., Tn.
3.Metabolomics tests are conducted in cell samples after simulation process of bacteria (or virus) invasion, T1, T2, ..., Tn, respectively, resulting in different zymograms as: M1, M2, ..., Mn. 3.The procedure of the zymograms analysis is described in the first article of metabolomics in this journal [1].
The other procedures are the same as described above. This comprehensive assessment of immunology is closer to the real situation of disease caused by multiple species of bacteria. Even if the pathology of host cells (such as cancerous blood cells of rat) is not caused by multiple species of invasive virus or bacteria (and by one species only), the invasive virus or bacteria of the same genetic strain may also evolve into various phenotypes in host body, which reflects the significance of comprehensive assessment of immunology.
Please note: if all the blood cells have been ‘eaten’ up (or no cell division rate) by a strain of bacteria during invasion simulation, then the value of this zymogram can be counted as zero for subsequent matrix calculation.
For the comprehensive assessment of immunology in host cells caused by the invasive virus or bacteria of the same genetic strain with different phenotypes:
The blood samples of a rat is abstracted and divided into two samples for the bio-signal simulation:
1.There are two kinds of cultivation conditions simulated in Lab for cell division: one is the ‘comfortable’ condition (Sample 1); the other is under electromagnetism simulation for cell cultivation (Sample 2); the cell samples are collected after sufficient cell division (Ten generations).
2.After sufficient cell division process, the electromagnetism simulation stops. Then both sample 1 and sample 2 are separately transferred into the simulation process of bacteria (or virus) invasion of the same genetic strain with different phenotypes: cells are cultivated individually and independently during the invasive simulation by different phenotypes of the same genetic bacteria (or virus) in Lab, and the invasion simulation process by different phenotypes of the same genetic bacteria (or virus) are labeled as T1, T2, ..., Tn.
3.Metabolomics tests are conducted (listed by article 1 of this journal) in cell samples after simulation process of bacteria (or virus) invasion, T1, T2, ..., Tn, respectively, resulting in different zymograms as: M1, M2, ..., Mn.The procedure of the zymograms analysis is described in the first article of metabolomics in this journal [1].
The other procedures are the same as described above. This electromagnetism simulation can be either constant electromagnetism fields or time-varying electromagnetic waves, which are further discussed later.
Conclusion:
The comprehensive assessment of immunology in host cells also provides indicators of training host cells by adjusting the parameters of biophysical simulation, once the specific zymograms, indicating the immunology against the specific invasive bacteria or virus (or the specific phenotype of an invasive pathogen), are identified by the methods presented in the other article of this journal. However, the higher dynamics, the better immunology against various pathogen species (or various phenotypes of a pathogen genotype).
This is the revised materials in book “Proceedings for Degree of Postgraduate Diploma in Environmental Science (3rd Edition).” Published in 2016. The ‘chapter’ content mentioned in this article is in previous book. Firstly Revised on 05/01/2021; Secondly Revised on 08/02/2021; Thirdly revised on 25/09/2021.
References:
[1].Liu Huan. Metabolomics (1) --- The Systematic Chemistry Fingerprints Between Genotype and Phenotype and its Application on the Conservation Genetics. Journal of Environment and Health Science. Feb. 2021.
|
|
发表于 2021-2-8 17:14:24