hliu092 发表于 2021-5-16 15:04:25

Discussion on Gene Sequencing and Classification of DNA Genetic Markers

This is the article 15 in the theme 'Environmental Physiology/环境生理学' of Journal of Environment and Health Science.

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hliu092 发表于 2021-8-27 15:21:27

Discussion on Gene Sequencing and Classification of DNA Genetic Markers

Author: Liu Huan (1983- ), Master of Science (First Class Honours), The University of Auckland

Nowadays, for the analysis of genetic similarity in species or subspecies by DNA sequencing technology, the selected DNA sequence fragments from gene bank only reveals a small part of the genetic information compared with the total genetic information contained by the whole genomes. Therefore, the significant difference derived from very limited/bias genetic information is likely to be a misleading conclusion. There are several reasons:

First, using DNA sequence fragment primers to amplify the chemical reaction, the information obtained is only the repetitive primer sequences of gene fragments and its relative molecular weighted among the total tested gene sequences in a experiment, but it does not reflect the spatial arrangement and combination information of various sequences on the genome. However, the arrangement and combination information of different gene sequences in the genomes is the main contribution factor of genetic variation, because most of the gene sequences revealed by DNA primers are highly conserved sequence fragments in the evolutionary process. Its own changes of DNA sequences are rare, which mainly depends on the change of arrangement and combination of various conserved sequences to achieve genetic variation in reproduction process for a population.

Secondly, in the past research, ovum cell and sperm cell are seldom separately distinguished to analyze each genetic contribution rate to their offspring. The mass/weight of DNA molecules in egg cells is much larger than DNA in sperm cells, so the genetic contribution rate of maternal DNA to offspring DNA is significantly higher than that of sperm suppliers. Consequently, statistic classification of genetic variation must input this statistic factor to distinguish. In comparison, the genetic contribution rate of bio electromagnetic waves (life signal waves) by sperm supplier to offspring is much higher than maternal parents (I have discussed this in biophysics articles).

Consequently, because the amount of genetic information tested by gene sequencing technology accounts for limited/bias proportion of the total genetic information of the genome, the significant difference in results reflected by multivariate statistics is likely to be a misleading conclusion.

For simple organisms such as viruses, because their reproduction process is only simple self-replication, and there is no complex arrangement and combination of gene recombination to achieve the genetic diversity for their offspring, such simple organisms can only rely on gene mutation to achieve the genetic variation for offspring. Therefore, the gene mutation rate of simple organisms such as viruses should be significantly higher than that of more advanced organisms. This is a common method for viruses to adapt to various environmental changes in population reproduction. This change includes both biotic and abiotic environmental conditions. However, it is expected that only a small proportion of occurrences in gene mutation may be directly related to the characteristics of virus invasive biology against host cells. Therefore, we should not overstate Big.




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hliu092 发表于 2021-5-16 15:05:15

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