hliu092 发表于 2021-2-5 17:25:29

DNA Marker (3): The Application of Spectral Informatics on the Genetic Marker

This is the article 1-3 in the theme 'Environmental Physiology/环境生理学' of Journal of Environment and Health Science.

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hliu092 发表于 2021-9-11 16:48:34

Article 1-3. The Application of Spectral Informatics on the Genetic Marker/光谱信息及其在遗传标记技术的应用 Author: Liu Huan (1983 - ), Master of Science (First Class Honours), The University of Auckland. This article proposed two new kinds of application methods of spectral informatics on the analysis of the DNA/RNA molecules. 1. In this book, DAPI fluorescence binding technology results in the appearance of AT rich region on chromosome, but the patterns of DAPI binding varies among different plant species. Consequently, this article presents the new method to observe the structure of DNA molecules at three dimensions: If the slide glass is the horizontal plane, and the vertical line is the eyesight line of microscope for DNA molecule observation, then the angle between the planes of AT DNA sequences and the eyesight line observed by microscope is ± α (0°≤ α ≤90°),and α is generally uniform in the DNA molecules of a species, but varies among different species. If this angle tends to be zero, then the DAPI binding tends to be not observed; If this angle tends to be 90°, then the DAPI binding tends to be more clearly observed by florescence microscope. The structure of DNA molecules can be consequently deduced by the clearness of fluorescence binding. 2. There is a novel Tech designed for future Research & Development in Air quality Monitoring: After a family of pathogenic virus (or bacteria) has been identified by the methodologies above, the unique SSR primers specifically for this family, which can not lead to PCR bands in the other microbial families but result in clear PCR bands in this pathogenic family only, are screened and synthesized into FISH probes for FISH step again. The methods of FISH probe preparation is listed . Then theconcentration of this pathogen family in water solution can be tested by ultraviolet spectrophotometer, yielding a feasible and affordable method for routine air quality monitoring. The steps are listed below. Please note, the specific SSR primer (or the specific locus), indicating the gene mutation bands of virus, is the key for this selection of FISH probe preparation. It is expected that the gene mutation virus family results in unusual and sharp increase of airborne density, as compared to its parental virus family, because the gene mutation significantly increases the genome replication rate. In this case, this specific SSR primer (or the specific locus), indicating the gene mutation bands of virus, may NOT be the unique one, but becomes the key to monitor the density of gene mutation virus. Step 1. In total five different densities of a virus family (such as the parental virus family of gene mutation one) are cultivated and separated in Lab. Step 2. Specific FISH probe is prepared for this virus family, and FISH procedure is conducted on five densities of this virus sample without the last drying process, leading to five different water solution concentrations (Sample 1, Sample 2 ..., Sample5) of virus genomes binding FISH probe. Step 3. The same volume of virus water solution are abstracted from Sample 1, Sample 2, ..., Sample 5, respectively, and the density of each virus water solution is counted by transmission electron microscopy after dying process. Step 4. The regression equation for ultraviolet spectrophotometer is consequently worked out by detecting the fluorescence intensity in five different water solution concentrations (Sample 1, Sample 2 ..., Sample 5) of virus genomes binding FISH probe. This is the revised materials in book “Proceedings for Degree of Postgraduate Diploma in Environmental Science (3rd Edition).” published in 2016. Firstly Revised on 03/01/2021; Secondly Revised on 05/02/2021. References:. 周延清, 张改娜与杨清香, 生物遗传标记与应用, 2008, 化学工业出版社.

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